Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F02%3A00006269" target="_blank" >RIV/00216224:14330/02:00006269 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography
Original language description
Micro axial tomography is a new method for microscopic image acquisition of fluorescence in situ hybridisation (FISH) stained cell nuclei with improved spatial resolution. Automated axial tomography has grown up from a common Brno-Heidelberg collaboration of two scientific groups, which are interested in the study of higher order chromatin structure in FISH stained cells using epifluorescence and confocal laser scanning fluorescence microscopy. The aim of the common Brno-Heidelberg project is to combinehigh-resolution cytometry (HRCM) and 2đ-tilting device in order to obtain statistically significant data about the structure of cell nuclei using advanced microscopy and fast automated computer image acquisition and analysis. In this study a method forthe preparation of cell nuclei attached to glass fibres has been developed. The optimised experimental procedure for the first time provides imaging of three-dimensionally conserved, FISH-stained cell nuclei fixed to a glass fibre
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2002
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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