Automated image analysis in fluorescence microscopy: From isolated cells to tissues and microarray images

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F04%3A00009701" target="_blank" >RIV/00216224:14330/04:00009701 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Automated image analysis in fluorescence microscopy: From isolated cells to tissues and microarray images

Original language description

Laboratory of Optical Microscopy has been working on the automation of image analysis in fluorescence microscopy since mid-1990s. At that time mostly 2D image analysis of FISH stained cell nuclei was explored. The images contained isolated cells (such asblood cells) that did not form many clusters and, therefore, image segmentation (the most difficult part of image analysis) was quite easy to automate. Later on 3D images of FISH (or immunofluorescence) stained cell nuclei acquired in confocal mode weredealt with. Also in this case the cell nuclei were well separated from each other, however somewhat blurred in axial direction due to the inferior resolution along z-axis. Also for this case automated methods have been developed including algorithms forthe computation of a mathematical model of cell (or cell nucleus) boundary in 3D. At the end of 1990s the necessity of tissue image analysis arose. In this area, fully automated image segmentation is not possible, hence the effort has be

Czech name

Automatizovaná analýza obrazu ve fluorescenční mikroskopii: Od izolovaných buněk k obrazům tkání a mikročipů

Czech description

Automatizovaná analýza obrazu ve fluorescenční mikroskopii: Od izolovaných buněk k obrazům tkání a mikročipů.

Type

D - Article in proceedings

CEP classification

JD - Use of computers, robotics and its application

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Biophysics of the Genome

ISBN

80-210-3560-9

ISSN

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e-ISSN

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Number of pages

3

Pages from-to

44-46

Publisher name

Masaryk University

Place of publication

Brno

Event location

Brno

Event date

Oct 12, 2004

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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