Precise 3D image alignment in micro-axial tomography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F03%3A00008610" target="_blank" >RIV/00216224:14330/03:00008610 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14330/03:00008544

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Precise 3D image alignment in micro-axial tomography

Original language description

Micro axial tomography is a challenging technique in microscopy which improves quantitative imaging especially in cytogenetic applications by means of defined sample rotation under the microscope objective. The advantage of micro-axial tomography is an effective improvement of the precision of distance measurements between point-like objects. Under certain circumstances, the effective (3D) resolution can be improved by optimized acquisition depending on subsequent, multi-perspective image recording of the same objects followed by reconstruction methods. This requires, however, a very precise alignment of the tilted views. We present a novel feature-based image alignment method with a precision better than the full width at half maximum of the point spread function. The features are the positions (centres of gravity) of all fluorescent objects observed in the images (e.g. cell nuclei, fluorescent signals inside cell nuclei, fluorescent beads, etc.). Thus, real alignment precision depend

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JD - Use of computers, robotics and its application

OECD FORD branch

—

Project

<a href="/en/project/IBS5004010" target="_blank" >IBS5004010: Development of new diagnostic techniques for oncology</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Microscopy

ISSN

0022-2720

e-ISSN

—

Volume of the periodical

209

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

17

Pages from-to

126

UT code for WoS article

—

EID of the result in the Scopus database

—