Topography of genetic loci in the nuclei of cells of colorectal carcinoma and adjacent tissue of colonic epithelium

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F04%3A00009648" target="_blank" >RIV/00216224:14330/04:00009648 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/04:00104661

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Topography of genetic loci in the nuclei of cells of colorectal carcinoma and adjacent tissue of colonic epithelium

Original language description

To determine the influence of increased gene expression and amplification in colorectal carcinoma on chromatin structure, the nuclear distances between pairs of bacterial artificial chromosome (BAC) clones with genomic separation from 800 to 29,000 kb were measured and compared between the tumor and parallel epithelial cells of six patients. The nuclear distances were measured between the loci in chromosomal bands 7p22.3-7p21.3; 7q35-7q36.3; 11p15.5-11p15.4; 20p13; 20p12.2; 20q11.21 and 20q12 where increased expression had been found in all types of colorectal carcinoma. The loci were visualized by three-dimensional fluorescence in situ hybridization using 22 BAC clones. Our results show that for short genomic separations, mean nuclear distance increases linearly with increased genomic separation. The results for some pairs of loci fell outside this linear slope, indicating the existence of different levels of chromatin folding. For the same genomic separations the nuclear distances we

Czech name

Topografie genetických lokusů v jádrech buněk kolorektálního karcionomu a přiléhající tkáně střevního epitelu

Czech description

Článek pojednává o topografii genetických lokusů v jádrech buněk kolorektálního karcionomu a přiléhající tkáně střevního epitelu

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chromosoma

ISSN

0009-5915

e-ISSN

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Volume of the periodical

112

Issue of the periodical within the volume

5

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

221

UT code for WoS article

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EID of the result in the Scopus database

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