In vivo imaging of cell interior using automated high-resolution cytometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F04%3A00009707" target="_blank" >RIV/00216224:14330/04:00009707 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/04:00108481
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
In vivo imaging of cell interior using automated high-resolution cytometry
Original language description
The fluorescent proteins are important innovation in the field of cell biology and in vivo experiments that use fluorescent proteins bring new interesting results from a different point of view than in vitro and in situ experiments. This presentation will introduce our automated 2D/3D high-resolution time-lapse image acquisition and analysis of living cells transfected with plasmids encoding fusion proteins or dyed with fluorescence probes. One of our in vivo projects, we will mention, is focused on thestudy of mitochondrial and nuclear apoptotic processes in living cells. Another in vivo project concentrates on the study of topography of telomeres and incidence of telomere-association phenomenon in various types of human tumor and healthy cells. In our experiments, cells are stably or transiently transfected with plasmid DNAs coding fusion mitochondrial apoptogenic or telomere-binding proteins with fluorescent proteins.
Czech name
In vivo snímání buněčného obsahu s využitím vysokorozlišovacího cytometru
Czech description
In vivo snímání buněčného obsahu s využitím vysokorozlišovacího cytometru

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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