Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F05%3A00013294" target="_blank" >RIV/00216224:14330/05:00013294 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells.
Original language description
Our project is focused on study of mitochondrial apoptogenic proteins released during apoptosis and associated with caspase-independent nuclear chromatin condensation and degradation. We introduce our automated 2D/3D/4D/FRET high-resolution image data acquisition and analysis system developed to visualize and quantify fluorescence signals in fixed and living cells. For our studies, we utilize the fluorescent proteins and various low-toxicity cell permeable fluorescent probes that make it possible to conduct the non-invasive quantitative visualization in living cells. In our experiments, cells are stably or transiently transfected or cotransfected by lipofection with DNA plasmids coding fusion proteins of mitochondrial apoptogenic proteins (cytochrom c,AIF, amid, and endonuclease g) with one of fluorescence proteins (EGFP, EYFP, or t-HcRed).
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů