Automated in vivo imaging of cell interior using fluorescent proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F04%3A00010822" target="_blank" >RIV/00216224:14330/04:00010822 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Automated in vivo imaging of cell interior using fluorescent proteins

Original language description

Techniques with fluorescent proteins have become very valuable tools for the study of cellular processes in living cells. These proteins enable the non-invasive quantitative visualization in vivo as molecular tags on natively non-fluorescent molecules ofinterest. Fluorescent proteins are welcome innovation in the field of cell biology, because in vivo experiments bring new interesting results and different point of view than in vitro and in situ experiments. This presentation will address automated 2D/3D high-resolution confocal image acquisition and analysis of cells stained with fluorescent proteins. While the approaches to automated in situ imaging of cell interior have been previously described by our group (Cytometry 45, 1-12, 2001), this contribution will concentrate on the modifications (both hardware and software ones) of our high-resolution image cytometers made for the purpose of automated in vivo imaging.

Czech name

Automatizované in vivo snímání buněčného obsahu s využitím fluorescenčních proteinů

Czech description

Automatizované in vivo snímání buněčného obsahu s využitím fluorescenčních proteinů.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GP204%2F03%2FD031" target="_blank" >GP204/03/D031: Apoptosis-inducing factor (AIF): its translocation from mitochondria and its action on nuclear chromatin</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cytometry

ISSN

0196-4763

e-ISSN

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Volume of the periodical

59A/2004

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

1

Pages from-to

107-107

UT code for WoS article

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EID of the result in the Scopus database

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