Single-cell image analysis of cellular localization and colocalization of endonuclease G, AIF and AMID in living cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F06%3A00020052" target="_blank" >RIV/00216224:14330/06:00020052 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Single-cell image analysis of cellular localization and colocalization of endonuclease G, AIF and AMID in living cells
Original language description
We studied the cellular localization and colocalization of proteins AIF, endonuclease G and AMID, which were previously found to be involved in caspase-independent apoptosis. We designed mammalian expression vectors, which carry genes encoding the proteins of interest fused to the fluorescent proteins to study cellular localization and colocalization of these proteins. We predicted the cellular localization of the proteins of interest using software, which analyses the aminoacid sequence of the proteinwith various algorithms. We also designed and applied a novel method of single-cell image analysis of the translocation of the fluorescent proteins into the nucleus. Using the listed methods, we managed to confirm by software prediction the localizationof AIF and endoG to mitochondria.
Czech name
—
Czech description
—
Classification
Type
O - Miscellaneous
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů