Relative expression of gammadelta T-cell receptor gene families detected by RT?PCR and capillary electrophoresis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F12%3A00059919" target="_blank" >RIV/00216224:14330/12:00059919 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/12:#0000957
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1744-313X.2012.01114.x/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/j.1744-313X.2012.01114.x/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1744-313X.2012.01114.x" target="_blank" >10.1111/j.1744-313X.2012.01114.x</a>

Result language
angličtina
Original language name
Relative expression of gammadelta T-cell receptor gene families detected by RT?PCR and capillary electrophoresis
Original language description
Gammadelta T cells are intensively studied because their function in infection, allergy, autoimmune disease, cancer and post-transplant period is not yet fully understood. PCR-based techniques were established to study the gamma variable (Vgamma) and delta variable (Vdelta) gene families. PCR product evaluation is routinely carried out by Southern blot analysis or the third complementarity-determining region (CDR3) spectratyping, but a fast and simple assessment of Vgamma and Vdelta gene family expression is missing. The aim of our study was to test capillary electrophoresis as a potential method for evaluating the composition of the gammadelta T-cell population. This report provides optimized PCR conditions for gammadelta T-cell receptor amplification. Further it describes the utilization of capillary electrophoresis in the Agilent 2100 Bioanalyzer to evaluate the relative expression of Vgamma and Vdelta gene families after their amplification.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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