Hybrid detectors improved time-lapse confocal microscopy of PML and 53BP1 nuclear body co-localization in DNA lesions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F13%3A00065961" target="_blank" >RIV/00216224:14330/13:00065961 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/13:00392608
Result on the web
<a href="http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8864223" target="_blank" >http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8864223</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S1431927612014353" target="_blank" >10.1017/S1431927612014353</a>
Alternative languages
Result language
angličtina
Original language name
Hybrid detectors improved time-lapse confocal microscopy of PML and 53BP1 nuclear body co-localization in DNA lesions
Original language description
We used hybrid detectors (HyDs) to monitor the trajectories and interactions of promyelocytic leukemia (GFP-PML) nuclear bodies (NBs) and mCherry-53BP1-positive DNA lesions. 53BP1 protein accumulates in NBs that occur spontaneously in the genome or in gamma-irradiationinduced foci. When we induced local DNA damage by ultraviolet irradiation, we also observed accumulation of 53BP1 proteins into discrete bodies, instead of the expected dispersed pattern. In comparison with photomultiplier tubes (PMTs), which are used for standard analysis by confocal laser scanning microscopy, HyDs significantly eliminated photobleaching of GFP and mCherry fluorochromes during image acquisition. The low laser intensities used for HyD-based confocal analysis enabled us toobserve NBs for the longer time periods, necessary for studies of the trajectories and interactions of PML and 53BP1 NBs.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP302%2F12%2FG157" target="_blank" >GBP302/12/G157: Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microscopy and Microanalysis
ISSN
1431-9276
e-ISSN
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Volume of the periodical
Volume 19
Issue of the periodical within the volume
Issue 2
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
360-369
UT code for WoS article
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EID of the result in the Scopus database
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