Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00464620" target="_blank" >RIV/68081707:_____/16:00464620 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14330/16:00087865 RIV/00216208:11110/16:10328049
Result on the web
<a href="http://dx.doi.org/10.1002/jcb.25551" target="_blank" >http://dx.doi.org/10.1002/jcb.25551</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jcb.25551" target="_blank" >10.1002/jcb.25551</a>

Result language
angličtina
Original language name
Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells
Original language description
We studied epigenetics, distribution pattern, kinetics, and diffusion of proteins recruited to spontaneous and -radiation-induced DNA lesions. We showed that PML deficiency leads to an increased number of DNA lesions, which was accompanied by changes in histone signature. In PML wt cells, we observed two mobile fractions of 53BP1 protein with distinct diffusion in spontaneous lesions. These protein fractions were not detected in PML-deficient cells, characterized by slow-diffusion of 53BP1. Single particle tracking analysis revealed limited local motion of 53BP1 foci in PML double null cells and local motion 53BP1 foci was even more reduced after -irradiation. However, radiation did not change co-localization between 53BP1 nuclear bodies and interchromatin granule-associated zones (IGAZs), nuclear speckles, or chromocenters. This newly observed interaction pattern imply that 53BP1 protein could be a part of not only DNA repair, but also process mediated via components accumulated in IGAZs, nuclear speckles, or paraspeckles. Together, PML deficiency affected local motion of 53BP1 nuclear bodies and changed composition and a number of irradiation-induced foci. J. Cell. Biochem. 117: 2583-2596, 2016. (c) 2016 Wiley Periodicals, Inc.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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