Automatic Quantification of Filopodia-Based Cell Migration
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F13%3A00068040" target="_blank" >RIV/00216224:14330/13:00068040 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1109/ISBI.2013.6556563" target="_blank" >http://dx.doi.org/10.1109/ISBI.2013.6556563</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1109/ISBI.2013.6556563" target="_blank" >10.1109/ISBI.2013.6556563</a>
Alternative languages
Result language
angličtina
Original language name
Automatic Quantification of Filopodia-Based Cell Migration
Original language description
We present a fully automatic approach to quantitatively analyze filopodia-based migration of fluorescent cells in 3D time-lapse series. The proposed method involves three steps. First, each frame of the time-lapse series is preprocessed using a steerablefilter and binarized to obtain a coarse segmentation of the cell shape. Second, a sequence of morphological filters is applied on the coarse binary mask to separate the cell body from individual filopodia. Finally, their length is estimated using a geodesic distance transform. The proposed approach is validated on 3D time-lapse series of lung adenocarcinoma cells. We show that the number of filopodia and their average length can be used as a descriptor to discriminate between different phenotypes of migrating cells.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
IN - Informatics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.3.30.0009" target="_blank" >EE2.3.30.0009: Employment of Newly Graduated Doctors of Science for Scientific Excellence</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
10th IEEE International Symposium on Biomedical Imaging
ISBN
9781467364546
ISSN
1945-7928
e-ISSN
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Number of pages
4
Pages from-to
668-671
Publisher name
IEEE
Place of publication
San Francisco
Event location
San Francisco
Event date
Apr 7, 2013
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000326900100167