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Model-Based Generation of Synthetic 3D Time-Lapse Sequences of Motile Cells with Growing Filopodia

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F17%3A00094698" target="_blank" >RIV/00216224:14330/17:00094698 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1109/ISBI.2017.7950644" target="_blank" >http://dx.doi.org/10.1109/ISBI.2017.7950644</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1109/ISBI.2017.7950644" target="_blank" >10.1109/ISBI.2017.7950644</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Model-Based Generation of Synthetic 3D Time-Lapse Sequences of Motile Cells with Growing Filopodia

  • Original language description

    The existence of benchmark datasets is essential to objectively evaluate various image analysis methods. Nevertheless, manual annotations of fluorescence microscopy image data are very laborious and not often practicable, especially in the case of 3D+t experiments. In this work, we propose a simulation system capable of generating 3D time-lapse sequences of single motile cells with filopodial protrusions, accompanied by inherently generated ground truth. The system consists of three globally synchronized modules, each responsible for a separate task: the evolution of filopodia on a molecular level, linear elastic deformation of the entire cell with filopodia, and generation of realistic, time-coherent cell texture. The capability of our system is demonstrated by generating a synthetic 3D time-lapse sequence of a single lung cancer cell with two growing filopodia, visually resembling its real counterpart acquired using a confocal fluorescence microscope.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

  • OECD FORD branch

    10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)

Result continuities

  • Project

    <a href="/en/project/GJ16-03909Y" target="_blank" >GJ16-03909Y: Development of Reliable Methods for Automated Quantitative Characterization of Cell Motility in Fluorescence Microscopy</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    14th IEEE International Symposium on Biomedical Imaging

  • ISBN

    9781509011728

  • ISSN

    1945-7928

  • e-ISSN

  • Number of pages

    5

  • Pages from-to

    822-826

  • Publisher name

    IEEE

  • Place of publication

    Melbourne

  • Event location

    Melbourne, Australia

  • Event date

    Jan 1, 2017

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article

    000414283200191