Quantification of the 3D Collagen Network Geometry in Confocal Reflection Microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F15%3A00080822" target="_blank" >RIV/00216224:14330/15:00080822 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1109/ICIP.2015.7351109" target="_blank" >http://dx.doi.org/10.1109/ICIP.2015.7351109</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1109/ICIP.2015.7351109" target="_blank" >10.1109/ICIP.2015.7351109</a>
Alternative languages
Result language
angličtina
Original language name
Quantification of the 3D Collagen Network Geometry in Confocal Reflection Microscopy
Original language description
The geometry of 3D collagen networks is a key factor that influences the behavior of live cells within extracellular matrices. This paper presents a hybrid, two-step method for fully automatic quantification of the 3D collagen network geometry at fiber resolution in confocal reflection microscopy images. First, a coarse binary mask of the entire network is obtained using steerable filtering and local Otsu thresholding. Second, individual collagen fibers are reconstructed by~tracing maximum ridges in theEuclidean distance map of the binary mask. The proposed method, validated in a novel framework using 3D collagen gels with various collagen and Matrigel concentrations, reveal that Matrigel affects the collagen network geometry by decreasing the networkpore size while preserving the fiber length and fiber persistence length.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
IN - Informatics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
22nd IEEE International Conference on Image Processing
ISBN
9781479983391
ISSN
1522-4880
e-ISSN
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Number of pages
4
Pages from-to
1791-1794
Publisher name
IEEE
Place of publication
Québec City
Event location
Québec City
Event date
Sep 27, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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