Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F15%3A00080823" target="_blank" >RIV/00216224:14330/15:00080823 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1109/EMBC.2015.7320283" target="_blank" >http://dx.doi.org/10.1109/EMBC.2015.7320283</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1109/EMBC.2015.7320283" target="_blank" >10.1109/EMBC.2015.7320283</a>

Result language
angličtina
Original language name
Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration
Original language description
The geometry of 3D collagen networks is a key factor that influences the behavior of live cells within extracellular matrices. This paper presents a method for automatic quantification of the 3D collagen network geometry with fiber resolution in confocalreflection microscopy images. The proposed method is based on a smoothing filter and binarization of the collagen network followed by a fiber reconstruction algorithm. The method is validated on 3D collagen gels with various collagen and Matrigel concentrations. The results reveal that Matrigel affects the collagen network geometry by decreasing the network pore size while preserving the fiber length and fiber persistence length. The influence of network composition and geometry, especially pore size,is preliminarily analyzed by quantifying the migration patterns of lung cancer cells within microfluidic devices filled with three different hydrogel types. The experiments reveal that Matrigel, while decreasing pore size, stimulates cell
Czech name
—
Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
ISBN
9781424492718
ISSN
1557-170X
e-ISSN
—
Number of pages
4
Pages from-to
8139-8142
Publisher name
IEEE
Place of publication
Milano
Event location
Milano
Event date
Aug 25, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000371717208103

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