Genomic Stability of the Cells during hiPSC Reprogramming and Endothelial Differentiation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F16%3A00088049" target="_blank" >RIV/00216224:14330/16:00088049 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Genomic Stability of the Cells during hiPSC Reprogramming and Endothelial Differentiation
Original language description
Studies of endothelial biology at genetic and molecular level are limited by the availability of relevant endothelial cells (ECs). Human induced pluripotent stem cells (hiPSCs) offer a potentially unlimited source of ECs. hiPSC-derived ECs (hiPSC-ECs) provide a robust and reproducible patient-specific model system for (1) tissue engineering, (2) drug development, (3) toxicity screening, and (4) disease modeling. However, one of the main concerns is the maintenance of genomic integrity of the cells throughout the processes of hiPSC reprogramming and differentiation in vitro. In our study we generated hiPSCs from various cell-type sources, including human umbilical vein ECs (HUVECs) and adult vein ECs. After phenotypical and functional characterization, hiPSC lines were differentiated into the hiPSC-ECs using previously published protocol (Orlova et al., 2014). Upon magnetic beads-based purification, the hiPSC-EC population displayed endothelial surface markers and functions consistent with primary ECs.
Czech name
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Czech description
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Project
<a href="/en/project/GBP302%2F12%2FG157" target="_blank" >GBP302/12/G157: Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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