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Correlation-based 2D Registration Method for Single Particle Cryo-EM Images

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F17%3A00102166" target="_blank" >RIV/00216224:14330/17:00102166 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1109/IPTA.2017.8310125" target="_blank" >http://dx.doi.org/10.1109/IPTA.2017.8310125</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1109/IPTA.2017.8310125" target="_blank" >10.1109/IPTA.2017.8310125</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Correlation-based 2D Registration Method for Single Particle Cryo-EM Images

  • Original language description

    The amount of image data generated in single particle cryo-electron microscopy (cryo-EM) is huge. This technique is based on the reconstruction of the 3D model of a particle using its 2D projections. The most common way to reduce the noise in particle projection images is averaging. The essential step before the averaging is the alignment of projections. In this work, we propose a fast 2D rigid registration approach for alignment of particle projections in single particle cryo- EM. We used cross-correlation in Fourier domain combined with polar transform to find the rotation angle invariant to the shift between the images. For translation vector estimation we used a fast version of upsampled image correlation. Our approach was evaluated on specifically created synthetic dataset. An experimental comparison with a a widely used in existing software iterative method has been performed. In addition, it was successfully applied to a real dataset from the Electron Microscopy Data Bank (EMDB).

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

  • OECD FORD branch

    10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Seventh International Conference on Image Processing Theory, Tools and Applications (IPTA), 2017

  • ISBN

    9781538618424

  • ISSN

    2154-512X

  • e-ISSN

  • Number of pages

    6

  • Pages from-to

    1-6

  • Publisher name

    IEEE

  • Place of publication

    Montreal

  • Event location

    Montreal, Canada

  • Event date

    Jan 1, 2017

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article

    000428743900049