Performance and Quality Comparison of Movie Alignment Software for Cryogenic Electron Microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14610%2F23%3A00136930" target="_blank" >RIV/00216224:14610/23:00136930 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2072-666X/14/10/1835" target="_blank" >https://www.mdpi.com/2072-666X/14/10/1835</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/mi14101835" target="_blank" >10.3390/mi14101835</a>
Alternative languages
Result language
angličtina
Original language name
Performance and Quality Comparison of Movie Alignment Software for Cryogenic Electron Microscopy
Original language description
Cryogenic electron microscopy (Cryo-EM) has been established as one of the key players in structural biology. It can reconstruct a 3D model of a sample at a near-atomic resolution. With the increasing number of facilities, faster microscopes, and new imaging techniques, there is a growing demand for algorithms and programs able to process the so-called movie data produced by the microscopes in real time while preserving a high resolution and maximal information. In this article, we conduct a comparative analysis of the quality and performance of the most commonly used software for movie alignment. More precisely, we compare the most recent versions of FlexAlign (Xmipp v3.23.03), MotionCor2 (v1.6.4), Relion MotionCor (v4.0-beta), Warp (v1.0.9), and CryoSPARC (v4.0.3). We tested the quality of the alignment using generated phantom data, as well as real datasets, comparing the alignment precision, power spectra density, and performance scaling of each program.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
IEEJ Transactions on Sensors and Micromachines
ISSN
2072-666X
e-ISSN
2072-666X
Volume of the periodical
14
Issue of the periodical within the volume
10
Country of publishing house
CH - SWITZERLAND
Number of pages
24
Pages from-to
1-24
UT code for WoS article
001095235000001
EID of the result in the Scopus database
2-s2.0-85175065572