FlexAlign: An Accurate and Fast Algorithm for Movie Alignment in Cryo-Electron Microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14610%2F20%3A00116269" target="_blank" >RIV/00216224:14610/20:00116269 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2079-9292/9/6/1040" target="_blank" >https://www.mdpi.com/2079-9292/9/6/1040</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/electronics9061040" target="_blank" >10.3390/electronics9061040</a>

Result language

angličtina

Original language name

FlexAlign: An Accurate and Fast Algorithm for Movie Alignment in Cryo-Electron Microscopy

Original language description

Cryogenic Electron Microscopy (Cryo-EM) has been established as one of the key players in Structural Biology. It can reconstruct a 3D model of the sample at the near-atomic resolution, which led to a Method of the year award by Nature, and the Nobel Prize in 2017. With the growing number of facilities, faster microscopes, and new imaging techniques, new algorithms are needed to process the so-called movies data produced by the microscopes in real-time, while preserving a high resolution and maximum of additional information. In this article, we present a new algorithm used for movie alignment, called FlexAlign. FlexAlign is able to correctly compensate for the shift produced during the movie acquisition on-the-fly, using the current generation of hardware. The algorithm performs a global and elastic local registration of the movie frames using Cross-Correlation and B-spline interpolation for high precision. We show that our execution time is compatible with real-time correction and that we preserve the high-resolution information up to high frequency.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)

Project

<a href="/en/project/EF16_013%2F0001802" target="_blank" >EF16_013/0001802: CERIT Scientific Cloud</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electronics

ISSN

2079-9292

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

6

Country of publishing house

CH - SWITZERLAND

Number of pages

25

Pages from-to

1-25

UT code for WoS article

000551113800001

EID of the result in the Scopus database

2-s2.0-85086840250