Cryo-EM and Single-Particle Analysis with Scipion
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F21%3A00129812" target="_blank" >RIV/00216224:14330/21:00129812 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.3791/62261" target="_blank" >http://dx.doi.org/10.3791/62261</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3791/62261" target="_blank" >10.3791/62261</a>
Alternative languages
Result language
angličtina
Original language name
Cryo-EM and Single-Particle Analysis with Scipion
Original language description
Cryo-electron microscopy has become one of the most important tools in biological research to reveal the structural information of macromolecules at near-atomic resolution. In single-particle analysis, the vitrified sample is imaged by an electron beam and the detectors at the end of the microscope column produce movies of that sample. These movies contain thousands of images of identical particles in random orientations. The data need to go through an image processing workflow with multiple steps to obtain the final 3D reconstructed volume. The goal of the image processing workflow is to identify the acquisition parameters to be able to reconstruct the specimen under study. Scipion provides all the tools to create this workflow using several image processing packages in an integrative framework, also allowing the traceability of the results. In this article the whole image processing workflow in Scipion is presented and discussed with data coming from a real test case, giving all the details necessary to go from the movies obtained by the microscope to a high resolution final 3D reconstruction. Also, the power of using consensus tools that allow combining methods, and confirming results along every step of the workflow, improving the accuracy of the obtained results, is discussed.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Jove-Journal of Visualized Experiments
ISSN
1940-087X
e-ISSN
—
Volume of the periodical
2021
Issue of the periodical within the volume
171
Country of publishing house
GB - UNITED KINGDOM
Number of pages
27
Pages from-to
1-27
UT code for WoS article
000658340400045
EID of the result in the Scopus database
2-s2.0-85108209058