Flexible workflows for on-the-fly electron-microscopy single-particle image processing using Scipion
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14610%2F19%3A00111213" target="_blank" >RIV/00216224:14610/19:00111213 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1107/S2059798319011860" target="_blank" >http://dx.doi.org/10.1107/S2059798319011860</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1107/S2059798319011860" target="_blank" >10.1107/S2059798319011860</a>
Alternative languages
Result language
angličtina
Original language name
Flexible workflows for on-the-fly electron-microscopy single-particle image processing using Scipion
Original language description
Electron microscopy of macromolecular structures is an approach that is in increasing demand in the field of structural biology. The automation of image acquisition has greatly increased the potential throughput of electron microscopy. Here, the focus is on the possibilities in Scipion to implement flexible and robust image-processing workflows that allow the electron-microscope operator and the user to monitor the quality of image acquisition, assessing very simple acquisition measures or obtaining a first estimate of the initial volume, or the data resolution and heterogeneity, without any need for programming skills. These workflows can implement intelligent automatic decisions and they can warn the user of possible acquisition failures. These concepts are illustrated by analysis of the well known 2.2 A resolution beta-galactosidase data set.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Crystallographica Section D
ISSN
2059-7983
e-ISSN
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Volume of the periodical
75
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
13
Pages from-to
882-894
UT code for WoS article
000497657100003
EID of the result in the Scopus database
2-s2.0-85072916357