Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F21%3A00122290" target="_blank" >RIV/00216224:14330/21:00122290 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-0716-1406-8_13" target="_blank" >http://dx.doi.org/10.1007/978-1-0716-1406-8_13</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-1406-8_13" target="_blank" >10.1007/978-1-0716-1406-8_13</a>
Alternative languages
Result language
angličtina
Original language name
Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods
Original language description
Cryo-electron microscopy has established as a mature structural biology technique to elucidate the three-dimensional structure of biological macromolecules. The Coulomb potential of the sample is imaged by an electron beam, and fast semi-conductor detectors produce movies of the sample under study. These movies have to be further processed by a whole pipeline of image-processing algorithms that produce the final structure of the macromolecule. In this chapter, we illustrate this whole processing pipeline putting in value the strength of "meta algorithms," which are the combination of several algorithms, each one with different mathematical rationale, in order to distinguish correctly from incorrectly estimated parameters. We show how this strategy leads to superior performance of the whole pipeline as well as more confident assessments about the reconstructed structures. The "meta algorithms" strategy is common to many fields and, in particular, it has provided excellent results in bioinformatics. We illustrate this combination using the workflow engine, Scipion.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)
Result continuities
Project
<a href="/en/project/EF16_013%2F0001802" target="_blank" >EF16_013/0001802: CERIT Scientific Cloud</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Structural Proteomics
ISBN
9781071614051
Number of pages of the result
33
Pages from-to
257-289
Number of pages of the book
344
Publisher name
Springer US
Place of publication
Clifton, N.J.
UT code for WoS chapter
000691017300014