The role of RNA Polymerase II contiguity and long-range interactions in the regulation of gene expression in human pluripotent stem cells.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F19%3A00107242" target="_blank" >RIV/00216224:14330/19:00107242 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1155/2019/1375807" target="_blank" >http://dx.doi.org/10.1155/2019/1375807</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2019/1375807" target="_blank" >10.1155/2019/1375807</a>
Alternative languages
Result language
angličtina
Original language name
The role of RNA Polymerase II contiguity and long-range interactions in the regulation of gene expression in human pluripotent stem cells.
Original language description
In this study, we examined long-range interactions between the POU5F1 gene and genes previously identified as being POU5F1 enhancer-interacting, namely, CDYL, TLE2, RARG, and MSX1 (all involved in transcriptional regulation), in human pluripotent stem cells (hPSCs) and their early differentiated counterparts. As a control gene, we used RUNX1, which is expressed during hematopoietic differentiation and not associated with pluripotency. To reveal how these long-range interactions between POU5F1 and the selected genes change with the onset of differentiation and upon RNAP II inhibition, we performed three-dimensional fluorescence in situ hybridization (3D-FISH) followed by computational simulation analysis. Our analysis showed that the numbers of long-range interactions between specific genes decrease during differentiation, suggesting that the transcription of monitored genes is associated with pluripotency. In addition, we showed that upon inhibition of RNAP II, long-range associations do not disintegrate and remain constant. We also analyzed the distance distributions of these genes in the context of their positions in the nucleus and revealed that they tend to have similar patterns resembling normal distribution. Furthermore, we compared data created in vitro and in silico to assess the biological relevance of our results.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Stem Cells International
ISSN
1687-966X
e-ISSN
1687-9678
Volume of the periodical
2019
Issue of the periodical within the volume
Article ID 1375807
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
1-12
UT code for WoS article
000459099600001
EID of the result in the Scopus database
2-s2.0-85065841485