5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F11%3A00049942" target="_blank" >RIV/00216224:14740/11:00049942 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/11:00367828

Result on the web

<a href="http://dx.doi.org/10.1007/s10858-011-9496-2" target="_blank" >http://dx.doi.org/10.1007/s10858-011-9496-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10858-011-9496-2" target="_blank" >10.1007/s10858-011-9496-2</a>

Result language

angličtina

Original language name

5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion

Original language description

Two novel 5D NMR experiments (CACONCACO, NCOCANCO) for backbone assignment of disordered proteins are presented. The pulse sequences exploit relaxation properties of the unstructured proteins and combine the advantages of 13C-direct detection, non-uniform sampling, and longitudinal relaxation optimization to maximize the achievable resolution and minimize the experimental time. The pulse sequences were successfully tested on the sample of partially disordered delta subunit from RNA polymerase from Bacillus subtilis. The unstructured part of this 20 kDa protein consists of 81 amino acids with frequent sequential repeats. A collection of 0.0003% of the data needed for a conventional experiment with linear sampling was sufficient to perform an unambiguousassignment of the disordered part of the protein from a single 5D spectrum.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biomolecular NMR

ISSN

0925-2738

e-ISSN

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Volume of the periodical

50

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

1-11

UT code for WoS article

000290044400001

EID of the result in the Scopus database

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