High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F12%3A00377502" target="_blank" >RIV/61388971:_____/12:00377502 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10858-012-9613-x" target="_blank" >http://dx.doi.org/10.1007/s10858-012-9613-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10858-012-9613-x" target="_blank" >10.1007/s10858-012-9613-x</a>

Result language
angličtina
Original language name
High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
Original language description
Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using delta subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
<a href="/en/project/GA204%2F09%2F0583" target="_blank" >GA204/09/0583: The Delta Subunit of RNA Polymerase from Gram positive Bacteria</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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