Selectivity among Two Lectins: Probing the Effect of Topology, Multivalency and Flexibility of ?Clicked? Multivalent Glycoclusters
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F11%3A00052848" target="_blank" >RIV/00216224:14740/11:00052848 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/chem.201002635" target="_blank" >http://dx.doi.org/10.1002/chem.201002635</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/chem.201002635" target="_blank" >10.1002/chem.201002635</a>
Alternative languages
Result language
angličtina
Original language name
Selectivity among Two Lectins: Probing the Effect of Topology, Multivalency and Flexibility of ?Clicked? Multivalent Glycoclusters
Original language description
We have designed a series of multivalent galactosylated glycoconjugates and studied their binding properties towards two lectins, from plant and bacterial origins, to determine their potential selectivity. The synthesis was achieved through copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) under microwave activation between propargylated multivalent scaffolds and an azido-functionalised carbohydrate derivative. The interactions of two galactose-binding lectins from Pseudomonas aeruginosa (PA-IL) and Erythrina cristagalli (ECA) with the synthesized glycoclusters were studied by hemagglutination inhibition assays (HIA), surface plasmon resonance (SPR) and isothermal titration microcalorimetry (ITC). The results obtained illustrate the influence of the scaffold's geometry on the affinity towards the lectin and also on the relative potency in comparison with a monovalent galactoside reference probe.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ME08008" target="_blank" >ME08008: Design of Carbohydrates and Glycomimetics as Antibacterial and Antiviral Drugs</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemistry - A European Journal
ISSN
0947-6539
e-ISSN
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Volume of the periodical
17
Issue of the periodical within the volume
7
Country of publishing house
DE - GERMANY
Number of pages
14
Pages from-to
2146-2159
UT code for WoS article
000287986500016
EID of the result in the Scopus database
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