In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F12%3A00057509" target="_blank" >RIV/00216224:14740/12:00057509 - isvavai.cz</a>
Result on the web
<a href="http://www.nature.com/emboj/journal/v31/n19/abs/emboj2012237a.html" target="_blank" >http://www.nature.com/emboj/journal/v31/n19/abs/emboj2012237a.html</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/emboj.2012.237" target="_blank" >10.1038/emboj.2012.237</a>
Alternative languages
Result language
angličtina
Original language name
In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination
Original language description
The Nrd1-Nab3-Sen1 (NNS) complex pathway is responsible for transcription termination of cryptic unstable transcripts and sn/snoRNAs. The NNS complex recognizes short motifs on the nascent RNA, but the presence of these sequences alone is not sufficientto define a functional terminator. We generated a homogeneous set of several hundreds of artificial, NNS-dependent terminators with an in vivo selection approach. Analysis of these terminators revealed novel and extended sequence determinants for transcription termination and NNS complex binding as well as supermotifs that are critical for termination. Biochemical and structural data revealed that affinity and specificity of RNA recognition by Nab3p relies on induced fit recognition implicating an a-helical extension of the RNA recognition motif.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
EMBO Journal
ISSN
0261-4189
e-ISSN
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Volume of the periodical
31
Issue of the periodical within the volume
19
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
3935-3948
UT code for WoS article
000309656600015
EID of the result in the Scopus database
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