Arabidopsis KINETOCHORE NULL2 Is an Upstream Component for Centromeric Histone H3 Variant cenH3 Deposition at Centromeres

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F13%3A00081916" target="_blank" >RIV/00216224:14740/13:00081916 - isvavai.cz</a>

Result on the web

<a href="http://www.plantcell.org/content/early/2013/09/05/tpc.113.114736.abstract" target="_blank" >http://www.plantcell.org/content/early/2013/09/05/tpc.113.114736.abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1105/tpc.113.114736" target="_blank" >10.1105/tpc.113.114736</a>

Result language

angličtina

Original language name

Arabidopsis KINETOCHORE NULL2 Is an Upstream Component for Centromeric Histone H3 Variant cenH3 Deposition at Centromeres

Original language description

The centromeric histone H3 variant cenH3 is an essential centromeric protein required for assembly, maintenance, and proper function of kinetochores during mitosis and meiosis. We identified a KINETOCHORE NULL2 (KNL2) homolog in Arabidopsis thaliana anduncovered features of its role in cenH3 loading at centromeres. We show that Arabidopsis KNL2 colocalizes with cenH3 and is associated with centromeres during all stages of the mitotic cell cycle, except from metaphase to mid-anaphase. KNL2 is regulatedby the proteasome degradation pathway. The KNL2 promoter is mainly active in meristematic tissues, similar to the cenH3 promoter. A knockout mutant for KNL2 shows a reduced level of cenH3 expression and reduced amount of cenH3 protein at chromocenters ofmeristematic nuclei, anaphase bridges during mitosis, micronuclei in pollen tetrads, and 30%seed abortion.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell

ISSN

1040-4651

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

9

Country of publishing house

US - UNITED STATES

Number of pages

16

Pages from-to

3389-3404

UT code for WoS article

000326287100020

EID of the result in the Scopus database

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