Targeted In Vivo Inhibition of Specific Protein?Protein Interactions Using Recombinant Antibodies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00074131" target="_blank" >RIV/00216224:14740/14:00074131 - isvavai.cz</a>
Result on the web
<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109875" target="_blank" >http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109875</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0109875" target="_blank" >10.1371/journal.pone.0109875</a>
Alternative languages
Result language
angličtina
Original language name
Targeted In Vivo Inhibition of Specific Protein?Protein Interactions Using Recombinant Antibodies
Original language description
With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated ?silencing? represents an intriguing alternative for the precise inhibition of a particular function of biomolecules.Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein?protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluatingdesired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLOS ONE : Public Library of Science
ISSN
1932-6203
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
"nestránkováno"
UT code for WoS article
000343941200081
EID of the result in the Scopus database
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