Gas-assisted annular microsprayer for sample preparation for time-resolved cryo-electron microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00079215" target="_blank" >RIV/00216224:14740/14:00079215 - isvavai.cz</a>

Result on the web

<a href="http://iopscience.iop.org/0960-1317/24/11/115001/pdf/0960-1317_24_11_115001.pdf" target="_blank" >http://iopscience.iop.org/0960-1317/24/11/115001/pdf/0960-1317_24_11_115001.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/0960-1317/24/11/115001" target="_blank" >10.1088/0960-1317/24/11/115001</a>

Result language

angličtina

Original language name

Gas-assisted annular microsprayer for sample preparation for time-resolved cryo-electron microscopy

Original language description

Time-resolved cryo-electron microscopy (TRCEM) has emerged as a powerful technique for transient structural characterization of isolated biomacromolecular complexes in their native state within the time scale of seconds to milliseconds. For TRCEM samplepreparation, a microfluidic device has been demonstrated to be a promising approach to facilitate TRCEM biological sample preparation. It is capable of achieving rapidly aqueous sample mixing, controlled reaction incubation, and sample deposition on electron microscopy (EM) grids for rapid freezing. One of the critical challenges is to transfer samples to cryo-EM grids from the microfluidic device. By using a microspraying method, the generated droplet size needs to be controlled to facilitate thin icefilm formation on the grid surface for efficient data collection, whilst not being so thin that it dries out before freezing, i.e. an optimized mean droplet size needs to be achieved.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF MICROMECHANICS AND MICROENGINEERING

ISSN

0960-1317

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

"nestránkováno"

UT code for WoS article

000345262800001

EID of the result in the Scopus database

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