Deveopment of a microfluidic specimenpreparation device for time-resolved cryo-electron microscopy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00094149" target="_blank" >RIV/00216224:14740/16:00094149 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Deveopment of a microfluidic specimenpreparation device for time-resolved cryo-electron microscopy
Original language description
The goal of structural biology is to be able to understand how macromolecular complex functions. In traditional, non-time-resolved cryoelectron microscopy (cryo-EM), this is accomplished by determination of static structures. Existing time-resolved techniques are difficult or inefficient, and thus have been rarely used. The goal of this proposal is to construct a customized cryo-preparation device specifically for the microfluidics-based time-resolved method that I previously helped develop. The steps required for time-resolved cryo-EM are, as quickly as possible: 1) to initiate a chemical reaction, 2) to form an aqueous layer thin enough to transmit the electron beam, and 3) to arrest the chemical reaction in cryogen. The microfluidics-based method makes use of a device which mixes two components, sprays the mixture onto an EM grid, and then plunges the grid into cryogen.
Czech name
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Czech description
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Publication year
2016
Confidentiality
C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.

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