Initial bridges between two ribosomal subunits are formed within 9.4 milliseconds, as studied by time-resolved cryo-EM
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00079214" target="_blank" >RIV/00216224:14740/14:00079214 - isvavai.cz</a>
Result on the web
<a href="http://www.pnas.org/content/111/27/9822.full.pdf+html" target="_blank" >http://www.pnas.org/content/111/27/9822.full.pdf+html</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.1406744111" target="_blank" >10.1073/pnas.1406744111</a>
Alternative languages
Result language
angličtina
Original language name
Initial bridges between two ribosomal subunits are formed within 9.4 milliseconds, as studied by time-resolved cryo-EM
Original language description
Association of the two ribosomal subunits during the process of translation initiation is a crucial step of protein synthesis. The two subunits (30S and 50S) of the bacterial 70S ribosome are held together by 12 dynamic bridges involving RNA-RNA, RNA-protein, and protein-protein interactions. The process of bridge formation, such as whether all these bridges are formed simultaneously or in a sequential order, is poorly understood. To understand such processes, we have developed and implemented a class of microfluidic devices that mix two components to completion within 0.4 ms and spray the mixture in the form of microdroplets onto an electron microscopy grid, yielding a minimum reaction time of 9.4 ms before cryofixation. Using these devices, we have obtained cryo-EM data corresponding to reaction times of 9.4 and 43 ms and have determined 3D structures of ribosomal subunit association intermediates.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.3.20.0042" target="_blank" >EE2.3.20.0042: Internationalisation of the Structural Biology Research Programme with Emphasis on New Directions in R&D</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
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Volume of the periodical
111
Issue of the periodical within the volume
27
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
9822-9827
UT code for WoS article
000338514800036
EID of the result in the Scopus database
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