A tRNA methyltransferase paralog is important for ribosome stability and cell division in Trypanosoma brucei

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00507442" target="_blank" >RIV/60077344:_____/16:00507442 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/16:43891110

Result on the web

<a href="https://www.nature.com/articles/srep21438.pdf" target="_blank" >https://www.nature.com/articles/srep21438.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/srep21438" target="_blank" >10.1038/srep21438</a>

Result language

angličtina

Original language name

A tRNA methyltransferase paralog is important for ribosome stability and cell division in Trypanosoma brucei

Original language description

Most eukaryotic ribosomes contain 26/28S, 5S, and 5.8S large subunit ribosomal RNAs (LSU rRNAs) in addition to the 18S rRNA of the small subunit (SSU rRNA). However, in kinetoplastids, a group of organisms that include medically important members of the genus Trypanosoma and Leishmania, the 26/28S large subunit ribosomal RNA is uniquely composed of 6 rRNA fragments. In addition, recent studies have shown the presence of expansion segments in the large ribosomal subunit (60S) of Trypanosoma brucei. Given these differences in structure, processing and assembly, T. brucei ribosomes may require biogenesis factors not found in other organisms. Here, we show that one of two putative 3-methylcytidine methyltransferases, TbMTase37 (a homolog of human methyltransferase- like 6, METTL6), is important for ribosome stability in T. brucei. TbMTase37 localizes to the nucleolus and depletion of the protein results in accumulation of ribosomal particles lacking srRNA 4 and reduced levels of polysome associated ribosomes. We also find that TbMTase37 plays a role in cytokinesis, as loss of the protein leads to multi-flagellated and multi-nucleated cells.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10605 - Developmental biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Volume of the periodical

6

Issue of the periodical within the volume

FEB 18 2016

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

21438

UT code for WoS article

000370347400001

EID of the result in the Scopus database

2-s2.0-84958576339