A tRNA methyltransferase paralog is important for ribosome stability and cell division in Trypanosoma brucei

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F16%3A43891110" target="_blank" >RIV/60076658:12310/16:43891110 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/16:00507442
Result on the web
<a href="http://www.nature.com/articles/srep21438" target="_blank" >http://www.nature.com/articles/srep21438</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/srep21438" target="_blank" >10.1038/srep21438</a>

Result language
angličtina
Original language name
A tRNA methyltransferase paralog is important for ribosome stability and cell division in Trypanosoma brucei
Original language description
Most eukaryotic ribosomes contain 26/28S, 5S, and 5.8S large subunit ribosomal RNAs (LSU rRNAs) in addition to the 18S rRNA of the small subunit (SSU rRNA). However, in kinetoplastids, a group of organisms that include medically important members of the genus Trypanosoma and Leishmania, the 26/28S large subunit ribosomal RNA is uniquely composed of 6 rRNA fragments. In addition, recent studies have shown the presence of expansion segments in the large ribosomal subunit (60S) of Trypanosoma brucei. Given these differences in structure, processing and assembly, T. brucei ribosomes may require biogenesis factors not found in other organisms. Here, we show that one of two putative 3-methylcytidine methyltransferases, TbMTase37 (a homolog of human methyltransferase- like 6, METTL6), is important for ribosome stability in T. brucei. TbMTase37 localizes to the nucleolus and depletion of the protein results in accumulation of ribosomal particles lacking srRNA 4 and reduced levels of polysome associated ribosomes. We also find that TbMTase37 plays a role in cytokinesis, as loss of the protein leads to multi-flagellated and multi-nucleated cells.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—

Project
<a href="/en/project/GJ15-21450Y" target="_blank" >GJ15-21450Y: Queuosine: The role of an essential tRNA modification in parasitic protist Trypanosoma brucei</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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