A virus capsid-like nanocompartment that stores iron and protects bacteria from oxidative stress
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00079216" target="_blank" >RIV/00216224:14740/14:00079216 - isvavai.cz</a>
Result on the web
<a href="http://emboj.embopress.org/content/embojnl/33/17/1896.full.pdf" target="_blank" >http://emboj.embopress.org/content/embojnl/33/17/1896.full.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15252/embj.201488566" target="_blank" >10.15252/embj.201488566</a>
Alternative languages
Result language
angličtina
Original language name
A virus capsid-like nanocompartment that stores iron and protects bacteria from oxidative stress
Original language description
Living cells compartmentalize materials and enzymatic reactions to increase metabolic efficiency. While eukaryotes use membrane-bound organelles, bacteria and archaea rely primarily on protein-bound nanocompartments. Encapsulins constitute a class of nanocompartments widespread in bacteria and archaea whose functions have hitherto been unclear. Here, we characterize the encapsulin nanocompartment from Myxococcus xanthus, which consists of a shell protein (EncA, 32.5 kDa) and three internal proteins (EncB, 17 kDa; EncC, 13 kDa; EncD, 11 kDa). Using cryo-electron microscopy, we determined that EncA self-assembles into an icosahedral shell 32 nm in diameter (26 nm internal diameter), built from 180 subunits with the fold first observed in bacteriophage HK97 capsid. The internal proteins, of which EncB and EncC have ferritin-like domains, attach to its inner surface. Native nanocompartments have dense iron-rich cores.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
EMBO Journal
ISSN
0261-4189
e-ISSN
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Volume of the periodical
33
Issue of the periodical within the volume
17
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
1896-1911
UT code for WoS article
000341839500008
EID of the result in the Scopus database
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