RBM7 subunit of the NEXT complex binds U-rich sequences and targets 3 '-end extended forms of snRNAs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00081028" target="_blank" >RIV/00216224:14740/15:00081028 - isvavai.cz</a>

Result on the web

<a href="http://nar.oxfordjournals.org/content/43/8/4236.full.pdf+html" target="_blank" >http://nar.oxfordjournals.org/content/43/8/4236.full.pdf+html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkv240" target="_blank" >10.1093/nar/gkv240</a>

Result language

angličtina

Original language name

RBM7 subunit of the NEXT complex binds U-rich sequences and targets 3 '-end extended forms of snRNAs

Original language description

The Nuclear Exosome Targeting (NEXT) complex is a key cofactor of the mammalian nuclear exosome in the removal of Promoter Upstream Transcripts (PROMPTs) and potentially aberrant forms of other noncoding RNAs, such as snRNAs. NEXT is composed of three subunits SKIV2L2, ZCCHC8 and RBM7. We have recently identified the NEXT complex in our screen for oligo(U) RNA-binding factors. Here, we demonstrate that NEXT displays preference for U-rich pyrimidine sequences and this RNA binding is mediated by the RNA recognition motif (RRM) of the RBM7 subunit. We solved the structure of RBM7 RRM and identified two phenylalanine residues that are critical for interaction with RNA. Furthermore, we showed that these residues are required for the NEXT interaction with snRNAs in vivo. Finally, we show that depletion of components of the NEXT complex alone or together with exosome nucleases resulted in the accumulation of mature as well as extended forms of snRNAs.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

43

Issue of the periodical within the volume

8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

4236-4248

UT code for WoS article

000355317200036

EID of the result in the Scopus database

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