A quantitative 14-3-3 interaction screen connects the nuclear exosome targeting complex to the DNA damage response
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F14%3A33150981" target="_blank" >RIV/61989592:15110/14:33150981 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1101/gad.246272.114" target="_blank" >http://dx.doi.org/10.1101/gad.246272.114</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1101/gad.246272.114" target="_blank" >10.1101/gad.246272.114</a>
Alternative languages
Result language
angličtina
Original language name
A quantitative 14-3-3 interaction screen connects the nuclear exosome targeting complex to the DNA damage response
Original language description
RNA metabolism is altered following DNA damage, but the underlying mechanisms are not well understood. Through a 14-3-3 interaction screen for DNA damage-induced protein interactions in human cells, we identified protein complexes connected to RNA biology. These include the nuclear exosome targeting (NEXT) complex that regulates turnover of noncoding RNAs termed promoter upstream transcripts (PROMPTs). We show that the NEXT subunit RBM7 is phosphorylated upon DNA damage by the MAPKAPK2 kinase and establish that this mediates 14-3-3 binding and decreases PROMPT binding. These findings and our observation that cells lacking RBM7 display DNA damage hypersensitivity link PROMPT turnover to the DNA damage response.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicine for regional development and human resources</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
GENES & DEVELOPMENT
ISSN
0890-9369
e-ISSN
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Volume of the periodical
28
Issue of the periodical within the volume
18
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
1977-1982
UT code for WoS article
000342277800001
EID of the result in the Scopus database
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