Atomic force microscopy for characterization of biomolecules, affinity complexes and cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00084143" target="_blank" >RIV/00216224:14740/15:00084143 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Atomic force microscopy for characterization of biomolecules, affinity complexes and cells
Original language description
The instrumentation and services available at the Core Facility of Nanobiotechnology in CEITEC MU will be introduced. The research possibilities will be demonstrated on experiments with proteins, nucleic acids, their affinity complexes and cells. The interaction of ssDNA binding protein with oligonucleotides was imaged using bare tips (Fig. 1), the binding forces in the affinity complex were studied using the protein ligand-modified tip and the ForceRobot AFM head for automated recording of force-distance curves. Similar experiments characterised immunoreactions between antibody and serum albumin and microbial cells as antigens and hybridisation of nucleic acids; interactions were confirmed using surface plasmon resonance and electrochemical measurements. Properties of mast cells were characterised in relation to the changes accompanying biotransformation events (Fig. 2).
Czech name
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Czech description
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Project
<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
XV. Workshop of Physical Chemists and Electrochemists
ISBN
9788021078574
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
38-39
Publisher name
Masaryk University
Place of publication
Brno
Event location
Brno
Event date
May 26, 2015
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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