TUT-DIS3L2 is a mammalian surveillance pathway for aberrant structured non-coding RNAs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00088170" target="_blank" >RIV/00216224:14740/16:00088170 - isvavai.cz</a>

Result on the web

<a href="http://emboj.embopress.org/content/early/2016/09/19/embj.201694857" target="_blank" >http://emboj.embopress.org/content/early/2016/09/19/embj.201694857</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15252/embj.201694857" target="_blank" >10.15252/embj.201694857</a>

Result language

angličtina

Original language name

TUT-DIS3L2 is a mammalian surveillance pathway for aberrant structured non-coding RNAs

Original language description

Uridylation of various cellular RNA species at the 3' end has been generally linked to RNA degradation. In mammals, uridylated pre-let-7 miRNAs and mRNAs are targeted by the 3' to 5' exoribonuclease DIS3L2. Mutations in DIS3L2 have been associated with Perlman syndrome and with Wilms tumor susceptibility. Using in vivo cross-linking and immunoprecipitation (CLIP) method, we discovered the DIS3L2-dependent cytoplasmic uridylome of human cells. We found a broad spectrum of uridylated RNAs including rRNAs, snRNAs, snoRNAs, tRNAs, vault, 7SL, Y RNAs, mRNAs, lncRNAs, and transcripts from pseudogenes. The unifying features of most of these identified RNAs are aberrant processing and the presence of stable secondary structures. Most importantly, we demonstrate that uridylation mediates DIS3L2 degradation of short RNA polymerase II-derived RNAs. Our findings establish the role of DIS3L2 and oligouridylation as the cytoplasmic quality control for highly structured ncRNAs.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

EMBO JOURNAL

ISSN

0261-4189

e-ISSN

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Volume of the periodical

35

Issue of the periodical within the volume

20

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

2179-2191

UT code for WoS article

000385708000003

EID of the result in the Scopus database

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