Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00088530" target="_blank" >RIV/00216224:14740/16:00088530 - isvavai.cz</a>

Result on the web

<a href="http://www.cell.com/molecular-plant/fulltext/S1674-2052(16)30191-5" target="_blank" >http://www.cell.com/molecular-plant/fulltext/S1674-2052(16)30191-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molp.2016.08.010" target="_blank" >10.1016/j.molp.2016.08.010</a>

Result language

angličtina

Original language name

Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components

Original language description

Auxin directs plant ontogenesis via differential accumulation within tissues depending largely on the activity of PIN proteins that mediate auxin efflux from cells and its directional cell-to-cell transport. Regardless of the developmental importance of PINs, the structure of these transporters is poorly characterized. Here, we present experimental data concerning protein topology of plasma membrane-localized PINs. Utilizing approaches based on pH-dependent quenching of fluorescent reporters combined with immunolocalization techniques,we mapped the membrane topology of PINs and further cross-validated our results using available topology modeling software. We delineated the topology of PIN1 with two transmembrane (TM) bundles of five a-helices linked by a large intracellular loop and a C-terminus positioned outside the cytoplasm. Using constraints derived from our experimental data, we also provide an updated position of helical regions generating a verisimilitude model of PIN1.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Plant

ISSN

1674-2052

e-ISSN

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Volume of the periodical

9

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

16

Pages from-to

1504-1519

UT code for WoS article

000389594100008

EID of the result in the Scopus database

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