ZD7288, a blocker of the HCN channel family, increases doubling time of mouse embryonic stem cells and modulates differentiation outcomes in a context-dependent manner

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00088770" target="_blank" >RIV/00216224:14740/16:00088770 - isvavai.cz</a>

Result on the web

<a href="http://springerplus.springeropen.com/articles/10.1186/s40064-016-1678-7" target="_blank" >http://springerplus.springeropen.com/articles/10.1186/s40064-016-1678-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s40064-016-1678-7" target="_blank" >10.1186/s40064-016-1678-7</a>

Result language

angličtina

Original language name

ZD7288, a blocker of the HCN channel family, increases doubling time of mouse embryonic stem cells and modulates differentiation outcomes in a context-dependent manner

Original language description

Pluripotent stem cells are the starting cell type of choice for the development of many cell-based regenerative therapies due to their rapid and unlimited proliferation and broad differentiation potential. The unique pluripotent cell cycle underlies both these properties. Hyperpolarization-activated cyclic nucleotide-gated cation (HCN) family channels have previously been reported to modulate mouse embryonic stem cell (ESC) proliferation and here we characterize the effects of HCN inhibitor ZD7288 on ESC proliferation and stem cell identity. The doubling time of cells treated with the HCN blocker increased by similar to 30 % due to longer G1 and S phases, resulting in a nearly twofold reduction in ESC numbers after 4 day serum-free culture. Slower progression through S phase was not accompanied by H2AX phosphorylation or cell stalling at transition points, although EdU incorporation in treated cells was reduced.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA15-20818S" target="_blank" >GA15-20818S: Targeting of therapy resistant glioma cancer stem cells - via ion channel control of nutrient transport</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

SpringerPlus

ISSN

2193-1801

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

January

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

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UT code for WoS article

000368875900004

EID of the result in the Scopus database

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