The map-based genome sequence of Spirodela polyrhiza aligned with its chromosomes, a reference for karyotype evolution

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00093897" target="_blank" >RIV/00216224:14740/16:00093897 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1111/nph.13592/abstract;jsessionid=45B89E4181F4C0B55B0226C67A998584.f04t02?systemMessage=Wiley+Online+Library+will+be+unavailable+on+Saturday+25th+March+from+07%3A00+GMT+%2F+03%3A00+EDT+%2F+15%3A00+SGT+for+4+hours+fo" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/nph.13592/abstract;jsessionid=45B89E4181F4C0B55B0226C67A998584.f04t02?systemMessage=Wiley+Online+Library+will+be+unavailable+on+Saturday+25th+March+from+07%3A00+GMT+%2F+03%3A00+EDT+%2F+15%3A00+SGT+for+4+hours+fo</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/nph.13592" target="_blank" >10.1111/nph.13592</a>

Result language
angličtina
Original language name
The map-based genome sequence of Spirodela polyrhiza aligned with its chromosomes, a reference for karyotype evolution
Original language description
Duckweeds are aquatic monocotyledonous plants of potential economic interest with fast vegetative propagation, comprising 37 species with variable genome sizes (0.158-1.88 Gbp). The genomic sequence of Spirodela polyrhiza, the smallest and the most ancient duckweed genome, needs to be aligned to its chromosomes as a reference and prerequisite to study the genome and karyotype evolution of other duckweed species. We selected physically mapped bacterial artificial chromosomes (BACs) containing Spirodela DNA inserts with little or no repetitive elements as probes for multicolor fluorescence in situ hybridization (mcFISH), using an optimized BAC pooling strategy, to validate its physical map and correlate it with its chromosome complement. By consecutive mcFISH analyses, we assigned the originally assembled 32 pseudomolecules (supercontigs) of the genomic sequences to the 20 chromosomes of S. polyrhiza. A Spirodela cytogenetic map containing 96 BAC markers with an average distance of 0.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/EE2.3.20.0189" target="_blank" >EE2.3.20.0189: Development of excellence in evolutionary cytogenomics, epigenetics and cell signalling</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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