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Limitation of current probe design for oligo-cross-FISH, exemplified by chromosome evolution studies in duckweeds

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F21%3A00548227" target="_blank" >RIV/60077344:_____/21:00548227 - isvavai.cz</a>

  • Result on the web

    <a href="https://link.springer.com/article/10.1007/s00412-020-00749-2" target="_blank" >https://link.springer.com/article/10.1007/s00412-020-00749-2</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s00412-020-00749-2" target="_blank" >10.1007/s00412-020-00749-2</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Limitation of current probe design for oligo-cross-FISH, exemplified by chromosome evolution studies in duckweeds

  • Original language description

    Duckweeds represent a small, free-floating aquatic family (Lemnaceae) of the monocot order Alismatales with the fastest growth rate among flowering plants. They comprise five genera (Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia) varying in genome size and chromosome number. Spirodela polyrhiza had the first sequenced duckweed genome. Cytogenetic maps are available for both species of the genus Spirodela (S. polyrhiza and S. intermedia). However, elucidation of chromosome homeology and evolutionary chromosome rearrangements by cross-FISH using Spirodela BAC probes to species of other duckweed genera has not been successful so far. We investigated the potential of chromosome-specific oligo-FISH probes to address these topics. We designed oligo-FISH probes specific for one S. intermedia and one S. polyrhiza chromosome (Fig. 1a). Our results show that these oligo-probes cross-hybridize with the homeologous regions of the other congeneric species, but are not suitable to uncover chromosomal homeology across duckweeds genera. This is most likely due to too low sequence similarity between the investigated genera and/or too low probe density on the target genomes. Finally, we suggest genus-specific design of oligo-probes to elucidate chromosome evolution across duckweed genera.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10603 - Genetics and heredity (medical genetics to be 3)

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Chromosoma

  • ISSN

    0009-5915

  • e-ISSN

    1432-0886

  • Volume of the periodical

    130

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    11

  • Pages from-to

    15-25

  • UT code for WoS article

    000607772200001

  • EID of the result in the Scopus database

    2-s2.0-85099458569