Limitation of current probe design for oligo-cross-FISH, exemplified by chromosome evolution studies in duckweeds
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F21%3A00548227" target="_blank" >RIV/60077344:_____/21:00548227 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s00412-020-00749-2" target="_blank" >https://link.springer.com/article/10.1007/s00412-020-00749-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00412-020-00749-2" target="_blank" >10.1007/s00412-020-00749-2</a>
Alternative languages
Result language
angličtina
Original language name
Limitation of current probe design for oligo-cross-FISH, exemplified by chromosome evolution studies in duckweeds
Original language description
Duckweeds represent a small, free-floating aquatic family (Lemnaceae) of the monocot order Alismatales with the fastest growth rate among flowering plants. They comprise five genera (Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia) varying in genome size and chromosome number. Spirodela polyrhiza had the first sequenced duckweed genome. Cytogenetic maps are available for both species of the genus Spirodela (S. polyrhiza and S. intermedia). However, elucidation of chromosome homeology and evolutionary chromosome rearrangements by cross-FISH using Spirodela BAC probes to species of other duckweed genera has not been successful so far. We investigated the potential of chromosome-specific oligo-FISH probes to address these topics. We designed oligo-FISH probes specific for one S. intermedia and one S. polyrhiza chromosome (Fig. 1a). Our results show that these oligo-probes cross-hybridize with the homeologous regions of the other congeneric species, but are not suitable to uncover chromosomal homeology across duckweeds genera. This is most likely due to too low sequence similarity between the investigated genera and/or too low probe density on the target genomes. Finally, we suggest genus-specific design of oligo-probes to elucidate chromosome evolution across duckweed genera.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chromosoma
ISSN
0009-5915
e-ISSN
1432-0886
Volume of the periodical
130
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
15-25
UT code for WoS article
000607772200001
EID of the result in the Scopus database
2-s2.0-85099458569