Analysis of Telomeric G -overhangs by in- Gel Hybridization

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00093962" target="_blank" >RIV/00216224:14740/16:00093962 - isvavai.cz</a>
Result on the web
<a href="http://www.bio-protocol.org/e1775" target="_blank" >http://www.bio-protocol.org/e1775</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.21769/BioProtoc.1775" target="_blank" >10.21769/BioProtoc.1775</a>

Result language
angličtina
Original language name
Analysis of Telomeric G -overhangs by in- Gel Hybridization
Original language description
Telomeric DNA in majority of eukaryotes consists of an array of TG-rich tandem repeats. The TG-rich DNA strand is oriented with its 3’ end towards chromosome termini and is usually longer than its complementary CA-rich strand, thus forming 3’ single stranded overhang (G-overhang). G-overhangs arise from incomplete replication of chromosome termini by the lagging strand mechanism and post-replicative nucleolytic processing. The G-overhang is important for telomere protection as it serves as a binding platform for specific proteins and is required for t-loop formation. Hence, structure of telomeric G-overhang is an important indicator of telomere maintenance and functionality. Here we describe a method for analysis of G-overhangs in a model plant Arabidopsis thaliana by in-gel hybridization technique. This method allows relative quantification of the amount of single stranded telomeric DNA.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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