Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00093963" target="_blank" >RIV/00216224:14740/16:00093963 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/protocol/10.1007%2F978-1-4939-3142-2_13" target="_blank" >http://link.springer.com/protocol/10.1007%2F978-1-4939-3142-2_13</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-3142-2_13" target="_blank" >10.1007/978-1-4939-3142-2_13</a>

Result language
angličtina
Original language name
Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis
Original language description
The ability to analyze cell division in both spatial and temporal dimensions within an organism is a key requirement in developmental biology. Specialized cell types within individual organs, such as those within shoot and root apical meristems, have often been identified by differences in their rates of proliferation prior to the characterization of distinguishing molecular markers. Replication-dependent labeling of DNA is a widely used method for assaying cell proliferation. The earliest approaches used radioactive labeling with tritiated thymidine, which were later followed by immunodetection of bromodeoxyuridine (BrdU). A major advance in DNA labeling came with the use of 5-ethynyl-2´deoxyuridine (EdU) which has proven to have multiple advantages over BrdU. Here we describe the methodology for analyzing EdU labeling and retention in whole plants and histological sections of Arabidopsis.
Czech name
—
Czech description
—

Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)