Epistatic and allelic interactions control expression of ribosomal RNA gene clusters in Arabidopsis thaliana

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F17%3A00095154" target="_blank" >RIV/00216224:14740/17:00095154 - isvavai.cz</a>

Result on the web

<a href="https://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1209-z" target="_blank" >https://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1209-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13059-017-1209-z" target="_blank" >10.1186/s13059-017-1209-z</a>

Result language

angličtina

Original language name

Epistatic and allelic interactions control expression of ribosomal RNA gene clusters in Arabidopsis thaliana

Original language description

Background: Ribosomal RNA (rRNA) accounts for the majority of the RNA in eukaryotic cells, and is encoded by hundreds to thousands of nearly identical gene copies, only a subset of which are active at any given time. In Arabidopsis thaliana, 45S rRNA genes are found in two large ribosomal DNA (rDNA) clusters and little is known about the contribution of each to the overall transcription pattern in the species. Results: By taking advantage of genome sequencing data from the 1001 Genomes Consortium, we characterize rRNA gene sequence variation within and among accessions. Notably, variation is not restricted to the pre-rRNA sequences removed during processing, but it is also present within the highly conserved ribosomal subunits. Through linkage mapping we assign these variants to a particular rDNA cluster unambiguously and use them as reporters of rDNA cluster-specific expression. We demonstrate that rDNA cluster-usage varies greatly among accessions and that rDNA cluster-specific expression and silencing is controlled via genetic interactions between entire rDNA cluster haplotypes (alleles). Conclusions: We show that rRNA gene cluster expression is controlled via complex epistatic and allelic interactions between rDNA haplotypes that apparently regulate the entire rRNA gene cluster. Furthermore, the sequence polymorphism we discovered implies that the pool of rRNA in a cell may be heterogeneous, which could have functional consequences.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

<a href="/en/project/GBP501%2F12%2FG090" target="_blank" >GBP501/12/G090: Evolution and Function of Complex Plant Genomes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

GENOME BIOLOGY

ISSN

1474-760X

e-ISSN

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Volume of the periodical

18

Issue of the periodical within the volume

May

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

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UT code for WoS article

000400331100001

EID of the result in the Scopus database

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