Formation of Staphylococcus aureus biofilm in the presence of sub-lethal concentrations of disinfectants: a transcriptomic analysis using RNA-seq

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F17%3A00098003" target="_blank" >RIV/00216224:14740/17:00098003 - isvavai.cz</a>

Result on the web

<a href="http://aem.asm.org/content/early/2017/10/09/AEM.01643-17" target="_blank" >http://aem.asm.org/content/early/2017/10/09/AEM.01643-17</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/AEM.01643-17" target="_blank" >10.1128/AEM.01643-17</a>

Result language

angličtina

Original language name

Formation of Staphylococcus aureus biofilm in the presence of sub-lethal concentrations of disinfectants: a transcriptomic analysis using RNA-seq

Original language description

Staphylococcus aureus is a common biofilm-forming pathogen. Low doses of disinfectants have previously been reported to promote biofilm formation and to increase virulence. The aim of this study was to use RNA-seq analysis to investigate global transcriptional changes in S. aureus in response to sub-lethal concentrations of commonly used food industry disinfectants (ethanol, Et; chloramine T, ChT; and their combination, Et_ChT), in order to better understand the effects of these agents on biofilm formation. Treatment with Et and Et_ChT resulted in more significantly altered expression profiles, compared to ChT. Our results revealed that Et and Et_ChT treatments enhanced the expression of genes responsible for regulation of gene expression (sigB), cell surface factors (clfAB), adhesins (sdrDE) and capsular polysaccharides (cap8EFGL) resulting in more intact biofilm. In addition, in this study we were able to identify the pathways involved in the adaptation of S. aureus to the stress of ChT treatment. Further, Et suppressed the effect of ChT on gene expression when these agents were used together at sub-lethal concentrations. These data show that in the presence of sub-lethal concentrations of tested disinfectants, S. aureus cells triggers protective mechanisms and tries to cope with them.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/LM2015043" target="_blank" >LM2015043: Czech Infrastructure for Integrative Structural Biology</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

APPLIED AND ENVIRONMENTAL MICROBIOLOGY

ISSN

0099-2240

e-ISSN

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Volume of the periodical

83

Issue of the periodical within the volume

24

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

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UT code for WoS article

000416937000008

EID of the result in the Scopus database

2-s2.0-85036469590