Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F17%3A00100438" target="_blank" >RIV/00216224:14740/17:00100438 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/content/pdf/10.1208%2Fs12249-016-0500-8.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1208%2Fs12249-016-0500-8.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1208/s12249-016-0500-8" target="_blank" >10.1208/s12249-016-0500-8</a>

Result language

angličtina

Original language name

Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering

Original language description

In this study, biodegradable poly(epsilon-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO2-i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO2-i-PCL due to collagen coating and incorporation of TiO2, respectively. EDX and XPS studies revealed distribution of entrapped TiO2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10305 - Fluids and plasma physics (including surface physics)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

AAPS PHARMSCITECH

ISSN

1530-9932

e-ISSN

—

Volume of the periodical

18

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

72-81

UT code for WoS article

000392104200009

EID of the result in the Scopus database

2-s2.0-84958769611