Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00100849" target="_blank" >RIV/00216224:14740/18:00100849 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/18:00502564

Result on the web

<a href="http://jcs.biologists.org/content/131/2/jcs202416" target="_blank" >http://jcs.biologists.org/content/131/2/jcs202416</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/jcs.202416" target="_blank" >10.1242/jcs.202416</a>

Result language

angličtina

Original language name

Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression

Original language description

Ribosomal RNA genes (rDNA) have been used as valuable experimental systems in numerous studies. Here, we focus on elucidating the spatiotemporal organisation of rDNA replication in Arabidopsis thaliana. To determine the subnuclear distribution of rDNA and the progression of its replication during the S phase, we apply 5-ethynyl-2'-deoxyuridine (EdU) labelling, fluorescence-activated cell sorting, fluorescence in situ hybridization and structured illumination microscopy. We show that rDNA is replicated inside and outside the nucleolus, where active transcription occurs at the same time. Nascent rDNA shows a maximum of nucleolar associations during early S phase. In addition to EdU patterns typical for early or late S phase, we describe two intermediate EdU profiles characteristic for mid S phase. Moreover, the use of lines containing mutations in the chromatin assembly factor-1 gene fas1 and wild-type progeny of fas1xfas2 crosses depleted of inactive copies allows for selective observation of the replication pattern of active rDNA. High-resolution data are presented, revealing the culmination of replication in the mid S phase in the nucleolus and its vicinity. Taken together, our results provide a detailed snapshot of replication of active and inactive rDNA during S phase progression.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Cell Science

ISSN

0021-9533

e-ISSN

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Volume of the periodical

131

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

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UT code for WoS article

000424842400004

EID of the result in the Scopus database

2-s2.0-85041181109