Visualization of the Nucleolus Using Ethynyl Uridine
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F18%3A00488387" target="_blank" >RIV/68081707:_____/18:00488387 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/18:00101536
Result on the web
<a href="http://dx.doi.org/10.3389/fpls.2018.00177" target="_blank" >http://dx.doi.org/10.3389/fpls.2018.00177</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fpls.2018.00177" target="_blank" >10.3389/fpls.2018.00177</a>
Alternative languages
Result language
angličtina
Original language name
Visualization of the Nucleolus Using Ethynyl Uridine
Original language description
Thanks to recent innovative methodologies, key cellular processes such as replication or transcription can be visualized directly in situ in intact tissues. Many studies use so-called click iT chemistry where nascent DNA can be tracked by 5-ethynyl-2'-deoxyuridine (EdU), and nascent RNA by 5-ethynyl uridine (EU). While the labeling of replicating DNA by EdU has already been well established and further exploited in plants, the use of EU to reveal nascent RNA has not been developed to such an extent. In this article, we present a protocol for labeling of nucleolar RNA transcripts using EU and show that EU effectively highlights the nucleolus. The method is advantageous, because the need to prepare transgenic plants expressing fluorescently tagged nucleolar components when the nucleolus has to be visualized can be avoided.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Plant Science
ISSN
1664-462X
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
FEB2018
Country of publishing house
CH - SWITZERLAND
Number of pages
8
Pages from-to
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UT code for WoS article
000425288800001
EID of the result in the Scopus database
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